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SMART Grids™| Usage and Handling Guide

SMART Grids are a new type of analysis platform for transmission electron microscopy that are designed to provide the highest combination of resolution, chemical compatibility, and chemical functionality of any available grid.

The following usage and handling guidelines will enable you to produce the highest quality images for your samples. These steps are also outlined in our downloadable Usage & Handling guide.

Care should be taken when handling grids to preserve the integrity of the SiO2 membrane windows. The windows are approx. 100 x 100 um squares with a thickness of 25 nm (for the standard design). The SiO2 membrane windows have been functionalized to enhance sample quality and to simplify sample preparation. Generally, samples are deposited on the membrane side of the grids.

Removing SMART Grids from sample holder box: Firmly grip the sides of the grid with high precision tweezers and twist as shown below. The grids will release with minimal effort. Attempting to pry or pull up the grid may damage it and is not recommended. You may also view our Usage Video for more instruction.

SMART Grid Gelpack Removal

Tweezer UsageClamping onto the region of grid without windows will ensure that none are damaged. The preferred methods are to clamp either the edge of the grids or the sides of the grids.

Aerosol UsageWhile not recommended, using a gas for removal of dust or other debris from surface of grid can be done at low velocity flow in the plane of the grid.

Tweezer UsageThe grids can be washed, soaked or coated with a variety of aqueous and organic solvents with no damage to the grid surface. When submerging, try not to move the grids in any direction perpendicular to the plane of the windows, but rather in directions parallel to the plane of the windows.

SMART Grids are NOT able to withstand sonication.